KMID : 0606920110190040438
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Biomolecules & Therapeutics 2011 Volume.19 No. 4 p.438 ~ p.444
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Phagocytic Effects of ¥â-Glucans from the Mushroom Coriolus versicolor are Related to Dectin-1, NOS, TNF-¥á Signaling in Macrophages
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Jang Seon-A
Kang Se-Chan Sohn Eun-Hwa
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Abstract
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The mushroom Coriolus versicolor contains biologically active polysaccharides, most of which belong to the ¥â-glucan group. Diverse physicochemical properties, due to different sources and isolated types of ¥â-glucans, can induce distinct biological activi-ties. We investigated the effects of ¥â-glucans from C. versicolor on phagocytic activity, nitric oxide (NO), TNF-¥á production, and signaling of dectin-1, a well-known ¥â-glucan receptor, in macrophages. ¥â-Glucans increased phagocytic activity and TNF-¥á and NO-iNOS/eNOS production. Laminarin, a specifi c inhibitor of dectin-1, showed strong inhibitory effects on phagocytosis and sub-sequent TNF-¥á, iNOS, and eNOS production increased by ¥â-glucans, indicating that ¥â-glucans reacts with dectin-1 receptors. We examined whether the aforementioned cytokines were involved in the signaling pathway from the dectin-1 receptor to phagocyto-sis, and found that the inhibition of iNOS, eNOS, and TNF-¥á receptors signifi cantly decreased ¥â-glucan-induced phagocytosis. In conclusion, our study showed that dectin-1 signaling, triggered by ¥â-glucans, subsequently elicited TNF-¥á and NO-iNOS/eNOS production, and that these molecules seem to act as secondary molecules that cause eventual phagocytosis by macrophages. These fi ndings suggest that C. versicolor could be used as a nutritional medicine that may be useful in the treatment of infectious disease.
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KEYWORD
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¥â-glucans, Coriolus versicolor, Dectin-1, Macrophage, NO, TNF-¥á
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